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| 詳細(xì)信息 規(guī)格 Gibco 11811-023 數(shù)量 常備現(xiàn)貨 說(shuō)明 瓶 產(chǎn)品詳細(xì)參數(shù)名稱：G-418 Sulfate (G418) 產(chǎn)品貨號(hào)：Gibco 11811-023 規(guī)格：5G 10G 產(chǎn)地： Gibco 品牌商標(biāo)： Gibco 供應(yīng)商：魯汶生物 數(shù)量：常備現(xiàn)貨 英文名：G-418 Sulfate 保存條件：Storage at C 詳細(xì)信息我公司提供的G418來(lái)自Gibco公司，貨號(hào)11811-023，技術(shù)參數(shù)如下： Shipping Conditi*** GENETICIN is shipped at ambient temperature. Storage Conditi*** GENETICIN should be stored at room temperature, 15 to 30°C. GENETICIN is soluble in water and should be stored at 2 to 8°C as a solution. Background GENETICIN is an aminoglycoside related to Gentamicin and is used as a selective agent in molecular genetics experiments. It is toxic to both prokaryotic and eukaryotic cells. The resistance genes are dominant and are located on both Transpos*** Tn601 (903) and Tn5. The gene on Tn5 is referred to as a aminoglycoside phosphotransferase 3' (I) or APH(3')I.The genes are bacterial in derivation but they can be expressed in eukaryotic cells. Efficient expression is achieved when the neo DNA sequence is linked to eukaryotic DNA sequences that permit transcription and processing of the Neo coding sequence into mRNA. Introduction of either of these genes into cells can confer resistance to GENETICIN, enabling the cells to grow in media containing GENETICIN. Description GENETICIN is also known as Antibiotic G-418 Sulfate. GENETICIN is supplied as a white powder. The amount of GENETICIN required to be present in culture media to select for resistant cells varies with a number of factors including cell type. Good laboratory practice requires that the optimal concentration of GENETICIN required to maintain and select cells must be determined for each set of growth conditi***. Invitrogen recommends that whenever experimental conditi*** are altered (including use of GENETICIN from a different lot), the optimal concentration of the product be re-evaluated. Quality Control Absorbance - Visible range: At a concentration of 100 mg/mL in water, GENETICIN gives a clear colorless solution. The absorbance of this solution at 400 nm is less than 0.075 O.D. Absorbance - Ultr***iolet range: At a concentration of 1 mg/mL in water, the absorbance at 280 nm is less than 0.015 O.D. 產(chǎn)品詳細(xì)描述產(chǎn)品促銷價(jià)格： 1g ￥450 5g ￥2000 |
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